adherence of K562 cells

Dennis Goode GOODE at ZOOL.UMD.EDU
Fri Apr 5 18:27:49 EST 1996


Victor,

Try polylysine or celltac an the coverslips before you plate out the 
cells.

-Dennis

  Victor Yankiwski <vy05+ at andrew.cmu.edu>
on  Fri,  5 Apr 1996 12:21:40 asked:

> Hi,
> 
> I'm trying to do SEM of K562 cells, but am having trouble with their
> non-adherence to both T.C. dishes and glass cover slips; aspirating
> their media before fixation would result in the loss of too many cells. 
> Treatment of the plates with collagen does not help much.  Any
> suggestions to make them stick, or regarding fixation for SEM would be
> appreciated. 
> 
> Thanks, 
> Victor Yankiwski
> vy05 at andrew.cmu.edu 
> 
> 



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