adherence of K562 cells

[Scott R. Burger]

Victor Yankiwski <vy05+ at andrew.cmu.edu> wrote:

>K562 cells ... having trouble with their non-adherence to both T.C. dishes and glass cover >slips Treatment of the plates with collagen does not help much.  

You might try coating the dishes and coverslips with polylysine.  This works so well that I use 
it as a positive control in adhesion assays.  

Good luck!

Scott R. Burger
Department of Laboratory Medicine and Pathology
University of Minnesota
burge009 at gold.tc.umn.edu