adherence of K562 cells

kevin kevins at erols.com
Tue Apr 9 22:14:59 EST 1996


Since K562 cells express alpha5 beta1 integrin, you will want to use
Fibronectin or GRGDSP peptide in order to get the cells to adhere.
Both can be obtained fromn Life Technologies

Kevin






On 8 Apr 1996 16:33:26 GMT, "Scott R. Burger"
<burge009 at gold.tc.umn.edu> wrote:

>Victor Yankiwski <vy05+ at andrew.cmu.edu> wrote:
>
>>K562 cells ... having trouble with their non-adherence to both T.C. dishes and glass cover >slips Treatment of the plates with collagen does not help much.  
>
>You might try coating the dishes and coverslips with polylysine.  This works so well that I use 
>it as a positive control in adhesion assays.  
>
>Good luck!
>
>Scott R. Burger
>Department of Laboratory Medicine and Pathology
>University of Minnesota
>burge009 at gold.tc.umn.edu
>
>
>




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