Permenant mounting of mRNA ish slides developed with BCIP/NBT

Wes Garrett wgarrett at ggpl.arsusda.gov
Wed Aug 14 15:52:29 EST 1996


How do you permenantly mount slides that have been prepared
for non-radioactive mRNA ISH using an alkaline phosphatase
conjugated antibody and NBT/BCIP as the chromogen.  I have 
tried brief alcohol dehydrations, air drying and xylene 
clearing with a permenant mounting medium.  In both cases,
I end up dissolving and precipitating the NBT/BCIP chromogen
into fine crystals.

Thanks

Wes Garrett
wgarrett at ggpl.arsusda.gov




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