Permenant mounting of mRNA ish slides developed with BCIP/NBT

[Wes Garrett]

How do you permenantly mount slides that have been prepared
for non-radioactive mRNA ISH using an alkaline phosphatase
conjugated antibody and NBT/BCIP as the chromogen.  I have 
tried brief alcohol dehydrations, air drying and xylene 
clearing with a permenant mounting medium.  In both cases,
I end up dissolving and precipitating the NBT/BCIP chromogen
into fine crystals.

Thanks

Wes Garrett
wgarrett at ggpl.arsusda.gov