G418 resistance lost... danger(s)?
dgk at zeus.ahabs.wisc.edu
Wed Aug 14 11:46:51 EST 1996
I assume you have a G418 resistant fibroblast cell line that was
established using a retroviral vector. Have the cells been grown in the
presence of G418 prior to the freeze-down?
I constructed fibroblast cell lines that express viral proteins along with
G418 resistance and were selected for stable expression. Even though the
cells have integrated copies of G418-resistance cassette, removal of
selection pressure is sufficient to cause a loss in expression.
Have a grreat day!
In article <1996Aug7.192319 at molbiol.ox.ac.uk>, rhubner at molbiol.ox.ac.uk wrote:
> hi there cellbiol-netters,
> some years ago a cell line was established in our lab under selection with
> G418 (retrovirus -> resistance)... mRNA studies were done on passages from
> DMSO-stocks, but several months ago we noticed that all freeze-downs are no
> longer gentamicin resistant...
> what can be expected from such cell lines regarding stability?
> thank you very much for comments!
> Have a nice day,
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