G418 resistance lost... danger(s)?

[Gopal Dasika]

I assume you have a G418 resistant fibroblast cell line that was
established using a retroviral vector.  Have the cells been grown in the
presence of G418 prior to the freeze-down? 
I constructed fibroblast cell lines that express viral proteins along with
G418 resistance and were selected for stable expression. Even though the
cells have integrated copies of G418-resistance cassette, removal of
selection pressure is sufficient to cause a loss in expression.

Have a grreat day!

Gopal.
 

In article <1996Aug7.192319 at molbiol.ox.ac.uk>, rhubner at molbiol.ox.ac.uk wrote:

> hi there cellbiol-netters,
> 
>  some years ago a cell line was established in our lab under selection with
> G418 (retrovirus -> resistance)... mRNA studies were done on passages from
> DMSO-stocks, but several months ago we noticed that all freeze-downs are no
> longer gentamicin resistant... 
>  what can be expected from such cell lines regarding stability? 
> 
>  thank you very much for comments!
> 
>  Have a nice day,
>  Roland