Need help with reducing western background...
milkman at postbox.acs.ohio-state.edu
Fri Aug 23 07:59:27 EST 1996
In article <rutka-2208961025080001 at 126.96.36.199> rutka at sickkids.on.ca (Dr. Jim Rutka) writes:
>Any suggestions on reducing background on western detections (HRPO -
>Chemiluminescence on PVDF membrane)?
>Dr. James T. Rutka rutka at sickkids.on.ca
>Assistant Professor, Neurosurgery Phone: (416)813-6425
>The Hospital for Sick Children Fax: (416)813-4975
>University of Toronto, CANADA
Decrease the amt of HRPO and biotinylated Ab. Worked wonders for me using
the B. M. Chemilum. kit. Had to determine this empirically however. I
ended up using a 1:20,000 diln of POD. good luck
amstutz.1 at osu.edu
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