Need help with reducing western background...

[milkman]

In article <rutka-2208961025080001 at 142.20.1.62> rutka at sickkids.on.ca (Dr. Jim Rutka) writes:



>Any suggestions on reducing background on western detections (HRPO -
>Chemiluminescence on PVDF membrane)?

>-- 
>Dr. James T. Rutka                             rutka at sickkids.on.ca
>Assistant Professor, Neurosurgery              Phone: (416)813-6425
>The Hospital for Sick Children                 Fax:   (416)813-4975
>University of Toronto, CANADA


Decrease the amt of HRPO and biotinylated Ab.   Worked wonders for me using 
the B. M. Chemilum. kit.    Had to determine this empirically however.  I 
ended up using a 1:20,000 diln of POD.  good luck

amstutz.1 at osu.edu