Need help with reducing western background...
dmthomas at orion.oac.uci.edu
Fri Aug 23 00:07:41 EST 1996
In article <rutka-2208961025080001 at 18.104.22.168>, rutka at sickkids.on.ca (Dr.
Jim Rutka) wrote:
> Any suggestions on reducing background on western detections (HRPO -
> Chemiluminescence on PVDF membrane)?
> Dr. James T. Rutka rutka at sickkids.on.ca
> Assistant Professor, Neurosurgery Phone: (416)813-6425
> The Hospital for Sick Children Fax: (416)813-4975
> University of Toronto, CANADA
a simple thing to do to avoid bad background in addition to lower the
concentration of the primary and the secondary Abs is to do all the washes
in TBS 1% Tween 20 containing 5 % non fat dry milk.
University of California, Irvine
College of Medicine
Dept. of Biological Chemistry
Med. Sci. I, Room D214
Irvine, CA 92717
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