Need help with reducing western background...

Didier Thomas dmthomas at
Fri Aug 23 00:07:41 EST 1996

In article <rutka-2208961025080001 at>, rutka at (Dr.
Jim Rutka) wrote:

> Any suggestions on reducing background on western detections (HRPO -
> Chemiluminescence on PVDF membrane)?
> -- 
> Dr. James T. Rutka                             rutka at
> Assistant Professor, Neurosurgery              Phone: (416)813-6425
> The Hospital for Sick Children                 Fax:   (416)813-4975
> University of Toronto, CANADA

Dear James,

a simple thing to do to avoid bad background in addition to lower the
concentration of the primary and the secondary Abs is to do all the washes

in TBS 1% Tween 20 containing 5 % non fat dry milk. 


Didier Thomas
University of California, Irvine
College of Medicine
Dept. of Biological Chemistry
Med. Sci. I, Room D214
Irvine, CA 92717

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