Need help with reducing western background...
Dr E. Buxbaum
EB15 at le.ac.uk
Tue Aug 27 05:38:18 EST 1996
dmthomas at orion.oac.uci.edu (Didier Thomas) wrote:
>a simple thing to do to avoid bad background in addition to lower the
>concentration of the primary and the secondary Abs is to do all the >washes in TBS 1% Tween 20 containing 5 % non fat dry milk.
If that does not help, 1% fish skin gelatine can be included. It does
give a cleaner blott, but is not very nice to work with.
More information about the Cellbiol