Need help with reducing western background...

Dr E. Buxbaum EB15 at le.ac.uk
Tue Aug 27 05:38:18 EST 1996


dmthomas at orion.oac.uci.edu (Didier Thomas) wrote:
>a simple thing to do to avoid bad background in addition to lower the
>concentration of the primary and the secondary Abs is to do all the >washes in TBS 1% Tween 20 containing 5 % non fat dry milk. 

If that does not help, 1% fish skin gelatine can be included. It does 
give a cleaner blott, but is not very nice to work with.




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