PBS - More or less ?!

"General account ", VENETTA flim at venetta.tran.wau.nl
Wed Aug 28 05:02:59 EST 1996


Hello,

I am currently setting up a procedure to label cells with fluorescent
probes. In such procedures, PBS is often used to wash the cells.
However, there are many recipes for PBS. Sometimes it only consists
of phosphate buffer, sometimes sodium chloride and/or potassium
chloride is added. Not only the composition but also the concentrations
differ.
Can somebody advise on the composition and concentrations of a PBS buffer
used for washing cells?!

Thanks for your time,

Joachim Goedhart
Dept. Biochemistry
University of Wageningen
The Netherlands




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