Cell labeling

Dennis J. Young djyoung at ucsd.edu
Mon Dec 2 17:17:24 EST 1996

In article <32A04B81.5F76 at compos.com.br>,
   pveras at COMPOS.COM.BR (patricia veras) wrote:
:Hi everybody, does anybody know what concentrations (and time) we can 
:use to label cells in culture (primary culture of lymphocytes 
:stimulated with a specific antigen) with Br/Fl-deoxiuridine. Thank you 
:very much.
:Patricia Veras
:Laboratory of Cellulars Pathology and Biology
:Bahia - Brazil
Try 0.5 to 1 hour and SEVERAL concentraions of BrdU (@ 10 micromolar).
Um, there are published methods in books and journals (in English).

* Dennis J. Young                            Voice : (619) 822-0407     *
* Flow Cytometry Core Facility               FAX   : (619) 822-0412     *
* University of California, San Diego  USA   e-mail: djyoung at ucsd.edu   *
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