DNA lebel

Scott Walsh swalsh at bme.jhu.edu
Sun Dec 8 16:53:29 EST 1996


Hi..
Can anyone tell me what is the best way to label a circular plasmid
(probably supercoiled) with radioactive label.  I tried nick translation
but the labeled mixture after passing through sephadex column and
running a gel seems to have all oligonucleotides.  I need to keep the
DNA as a circular plasmid. Thanks..
Please reply at kroy at bme.jhu.edu

Thanks..

Krish Roy



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