Dr E. Buxbaum
EB15 at le.ac.uk
Tue Dec 10 08:49:10 EST 1996
brackena at tcd.ie (Adrian Bracken) wrote:
>Is it possible to run samples on SDS gel and then cut out and sequence >the protein bands that are present in one lane and not in another?
This is a standard technique: Separate by one- or twodimensional
electrophoresis, western-blot onto PVDF membranes, stain with Coomassie
blue or Ponceau red, cut out the interesting bands and put them (still on
the PVDF) into a gas phase sequencer.
You have to use PVDF membranes though, nitrocellulose does not work.
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