lysis buffer for monolayer culture + ELISA

Dr. O. Pullig oliver.pullig at rzmail.uni-erlangen.de
Wed Dec 18 09:24:27 EST 1996


HI,
I´m doing monolayer cultures of chondrocytes and want to lyse the cells
and also get the extracellular matrix in solution for quantitation of
collagens with inhibition ELISA. Therefore the buffer should be
- able to lyse the cells and bring also the matrix in solution
- work with ELISA 
- should be able get concentrated in centricons

any suggestions

Oliver



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