Electroporation of mammalian cells

Christian Reiser christian.reiser at rzmail.uni-erlangen.de
Fri Feb 2 21:50:46 EST 1996


Hi all!

I'm searching for methods for electroporation of renal mesangial cells - a
kind of smooth muscle cells.
My aim is to get antibodies into these cells. At the moment I have two
possibilities : cell death or living cells without any antibody inside
:-(  .
So, does anybody know a good protocol. I have an electroporator from
HOEFER and use cuvette electrodes with 2mm spacing.
If anybody knows a better method - I would also be lucky.
Thanks
               Chris



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