How to load pertussis toxin

Klaus Rytved krytved at
Tue Feb 6 20:40:02 EST 1996

I want to reverse a Gi protein action on cAMP response with pertussis toxin 
(PT) in epithelial cells.
Does any have a good suggestion on how to load PT into the  cells.
I have tried to incubate with 0.5-1 mg/ml PTX in the media for several hours 
without any luck.

Is there any drugs or protocols that will enhance the uptake of PTX ?
How do I make sure that PT is loaded if it doesnt inhibit the G-protein ?

Any suggestions are welcome.

Klaus Rytved

krytved at

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