bacterial housekeeping genes
Michael C. Gorry
mcgorry at med.pitt.edu
Thu Feb 8 13:36:40 EST 1996
I have been looking for a bacterial housekeeping gene that has
a high level of expression. Would GADPH be suitable? We are
looking for any gene that would have a high mRNA level within
a cell. I haven't been able to find anything published that discusses
expression rates within cells. There was, however, a mention
of using GADPH for an RT-PCR quantitative control. Is anyone
aware of other "controls" that have been used to quantitate PCR
reactions? I would appreciate any advice.
Thanks
--
Michael Gorry
mcgorry at med.pitt.edu
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