PVDF

[Dr E. Buxbaum]

klennon at acs.bu.edu wrote:
> I am in the process of setting up Westerns in my lab and am curious if I can 
> transfer my proteins onto PVDF one day and do the blocking/immunodetection the 
> following day. 

This should work without problems, blotts on PVDF can be stored air dried for a long time.
Just remember to wett them in methanol and rinse them in bidist. water and then PBS before 
further processing them.