Cell culture endocytosis
computer at mail.teleport.com
Wed Feb 14 18:42:43 EST 1996
Nigel Kenward wrote:
> I'm trying to get proteins and peptides into cells which have a poorly developped
> endocytic system. Has anyone got a method for this ?
> My guess is that I can maximise fluid phase endocytosis and pinocytosis by
> serum-deprivation folloed by the addition of higher serum mixed with my protein
> and peptide, but I'd appreciate any advice / refs which give details on this type
> of thing.
> Thanks in anticipation,
Have you considered loading them in liposome vesicles. There are
published protocols for doing this to get antibodies and other
macromolecules into cells non invasively.
More information about the Cellbiol