Cell culture endocytosis

Computer computer at mail.teleport.com
Wed Feb 14 18:42:43 EST 1996


Nigel Kenward wrote:
> 
> Hi,
> I'm trying to get proteins and peptides into cells which have a poorly developped
> endocytic system. Has anyone got a method for this ?
> My guess is that I can maximise fluid phase endocytosis and pinocytosis by
> serum-deprivation folloed by the addition of higher serum mixed with my protein
> and peptide, but I'd appreciate any advice / refs which give details on this type
> of thing.
> Thanks in anticipation,
> Nigel

Have you considered loading them in liposome vesicles.  There are 
published protocols for doing this to get antibodies and other 
macromolecules into cells non invasively.

Ian



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