charcoal stripped serum
colin at fungus.biochem.ualberta.ca
Wed Feb 14 23:37:42 EST 1996
rs690935 at condor.mbcr.bcm.tmc.edu (ramiro salas) wrote:
>Hi, you all people,
>I got a question. I need to culture some primary neurons in a thyroid
>hormone free media. Can I do it by stripping the serum with charcoal? Is
>there any reference on how to do that?
Try getting in touch with someone in O'Malley's group in Cell Biology at Baylor.
Someone there must know how to do that. My recollection when we did was to add
activated charcoal (Norit-A) to 5% (w/v) and then heat to 50°C for 30 minutes,
followed by filtration to remove the charcoal.
>Thanks in advance,
>rs690935 at condor.mbcr.bcm.tmc.edu
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