rare cell isolation
Condie Carmack
ccarmack at genweb.com
Thu Feb 15 14:27:18 EST 1996
Eberhard-
The systems you mentioned are very good for enrichment but if
you need to get pure populations of cells, I would suggest you
looking to flow cytometry as a final step. The columns work
well as a first step to enrich, but in a lot of cases, that is
not good enough. If you are looking for proteins that are
secreted there is a new technique that encapsulates the cells
in agarose then traps the secreted protein and is detected
with an antibody. You can sort the cell and recover it alive.
It is possible to use this technique to sort one in a million
or more. (IF YOU HAVE A GOOD ANTIBODY!) If you would like more
details, please write me back.
Condie Carmack
GenWeb
ccarmack at genweb.com
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