Mycoplasma treatment of cell cultures

Eric DELABESSE delabess at infobiogen.fr
Thu Feb 22 10:42:26 EST 1996


Help, dear netters!!!!!!!

Our cell cultures are contaminated with mycoplamas. After 4 weeks of
treatment with tiamuline (4.3 µg/ml), doxicycline (4.4 µg/ml) and
lincomycine (27 µg/ml), we have still detected mycoplasmas by PCR method.
Cell cultures which were successfully treated (negative PCR), again were
positive by PCR one month later.

Is there a better treatment? What can we do?

Thanks sincerely for your help!

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DELABESSE Eric                PHONE : (33-1) 44-49-06-75
Unité CNRS URA 1461           FAX : (33-1) 44-49-06-76
"Médiateurs de la régulation  e-mail:delabess at infobiogen.fr
des systèmes lymphoïdes et 
hématopoiétiques"
Universite Paris V                     
Hopital NECKER
149-161, rue de Sevres
75743 Paris cedex 15, FRANCE
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