charcoal stripped serum

Colin Rasmussen colin at fungus.biochem.ualberta.ca
Fri Feb 23 20:59:21 EST 1996


DANIEL Y KIM wrote:
> 
> Re: stripped serum preparation.
> 
> Just a bit of advice:  The fines of activated charcoal are really a pain
> to remove by filtration (serum + carbon clogs filters).  You would be
> well advised to consider pre-filters and also, remove fines from the
> charcoal before you begin.  You might want to pellet the carbon in a
> centrifuge and wash the pellet a few times.  In this way, you can remove
> the bulk of the carbon from the serum by centrifugation, followed by
> filtration to sterilize.
> 
> Daniel Kim

Paper filters. Usually Whatman #1 does a pretty good job.



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