charcoal stripped serum
colin at fungus.biochem.ualberta.ca
Fri Feb 23 20:59:21 EST 1996
DANIEL Y KIM wrote:
> Re: stripped serum preparation.
> Just a bit of advice: The fines of activated charcoal are really a pain
> to remove by filtration (serum + carbon clogs filters). You would be
> well advised to consider pre-filters and also, remove fines from the
> charcoal before you begin. You might want to pellet the carbon in a
> centrifuge and wash the pellet a few times. In this way, you can remove
> the bulk of the carbon from the serum by centrifugation, followed by
> filtration to sterilize.
> Daniel Kim
Paper filters. Usually Whatman #1 does a pretty good job.
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