immunoprecipitations in thymocytes....

[Stacy Ferguson]

In article <mrtour-2202961410070001 at ski-h-99-098.ski.mskcc.org>,
michelle or doug...... <mrtour at stud.med.cornell.edu,dburtrum at ski.mskcc.org> wrote:
>Hello:
>
>I am embarking upon some experiments in which i will be doing ips on
>thymocytes....
>
>does anyone have some recipes for lysis buffers which work with these cells?


There is a recipe for RIPA buffer in Maniatis (the newer, 3 volume edition)
that works well for thymic immunoprecipitations in my hands.


Stacy