EtOH + PBS = precipitation?

Colin Rasmussen colin at fungus.biochem.ualberta.ca
Tue Feb 27 22:48:25 EST 1996


LU Pin wrote:
> 
> Recently when I tried to prepare 95% ethanol in PBS, I always got the
> same problem. When PBS was added into abs. ethanol, the solution became
> turbid. Later, some white precipitate formed. Shaking or vortexing during
> PBS addition did not help. The PBS I used contains NaCl, KCl, Na2HPO4,
> KH2PO4. Anyone knows what's wrong or how to avoid this? Thank you very much.

Sounds  like the PO4 is precipitating. What is the final % EtOH you 
are trying to achieve, and why have you decided on PBS as opposed to 
other buffers.

Colin



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