Peptide immobilisation - Spacer length?

Michael Daws Michael.Daws at
Thu Jan 4 10:33:32 EST 1996

I am going to attach a peptide to an aminoalkyl agarose support using a
bifunctional cross linker - does anyone know what the best spacer length
would be to limit steric hindrance.  Presumably if the hydrophobic
spacer is too long it will allow increased non-specific binding or
spacer cleavage?


Mike Daws

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