Peptide immobilisation - Spacer length?

Michael Daws Michael.Daws at ucmp.umu.se
Thu Jan 4 10:33:32 EST 1996


I am going to attach a peptide to an aminoalkyl agarose support using a
bifunctional cross linker - does anyone know what the best spacer length
would be to limit steric hindrance.  Presumably if the hydrophobic
spacer is too long it will allow increased non-specific binding or
spacer cleavage?

Thanks.

Mike Daws





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