ca2+ fluorescence

Andreas Simm Simm at vax.rz.uni-wuerzburg.d400.de
Mon Jan 8 16:14:09 EST 1996


Dear Netters,
I am trying to measure intracellular Ca2+ using fura2-AM with a Fluoromax from 
SPEX.
Does anybody have any experience with a Fluoromax measuring Ca2+?

Is the "multigroup scan" the appropriate program for measurements?
When calibrating the maximal cellular response, addition of detergents leads 
to an increase in fluorescence as exspected, but after addition of 
EDTA a further increase is observed. 
Can anybody comment on that?

Monika Leicht



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