How is Agar prepared?

schelber at mailer.uni-marburg.de schelber at mailer.uni-marburg.de
Mon Jan 8 12:55:25 EST 1996


What do you whant to do?

Growing of E.coli?

The standard Medium for growing E.coli is LB(Lura Bertani):

1l Medium contains:

10g bacto-tryptone
 5g bacto yest-extract
10g NaCl2

for Agar-Plates ad.

15g Agar

ad deionized or destilled water to 1l.

Autoclave for 25 min at 123°C.

Agar-plates are prepared at approx. 50-60°C in
sterile petri-dishes.

Agar, yeast-extract and tryptone are available from
DIFCO LABORATORIES, Detroit Michigan USA.

Litrature:

Sambrook,J; Fritsch; E.F. and Maniatis, T. (1989)
Molecular Cloning - A Laboratory Manual
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York





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