Dr John Paul Cassella
j.cassella at ic.ac.uk
Thu Jan 11 13:17:53 EST 1996
There are antibiotics certainly worth trying before turning to an animal
However, it would be useful to know the circumstances of this infection -
just one cell line, a whole lab, or new in-coming material. Do you have
spare vials of non-contaminated cells in reserve?
I have personal experience of the ICN antibiotic abreviated to MRA, which
is in fact a quinolone derivative. 200ul per 10 ml culture medium, 3x per
week, for two weeks, removed the agent permentantly. I tested the
supernatant with a gene-probe test kit for mycoplasma DNA (don't have the
name at present but can get it if you want). Also, ciprofloxacin
(ciproxin) from Bayer is supposedly as effective as MRA, and probably a
Good luck. I'd like to hear how you get on.
PS. My e-mail address is j.dye at ic.ac.uk
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