PC12 cells-passages
Marek Treiman
mat at biobase.dk
Wed Jul 3 06:28:14 EST 1996
Hello netters,
What does the passage number for a batch of PC12s really mean? If you
received a batch of cells from someone designated "passage 10", do you
keep increasing this number or do you start from the beginning? The
latter alternative seems to make little sense biologically - but on the
other hand, if a continuing, "absolute" passage count were to be
maintained, it would have to go all the way back to Greene and Tischler's
work establishing PC12 line! Or do people set the number to "0" each time
they freeze the cells - which again would not reflect their true "age"?!
Enlightened and enlightening comments very appreciated!! Cheers, Marek
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