Why is it necessary to inactivate serum ?

CW Tuk cw.tuk.cell at med.vu.nl
Thu Jul 11 08:52:35 EST 1996


In article <4s2fqa$bo5 at sci3.sri.ucl.ac.be>, verhelpe <Verhelpen at orto.ucl.ac.be> says:
>
>Hello,
>I wonder why is it necessery to inactivate serum by heat for cellular 
>culture ?
>Thanks
>
>
>Anne Jungbluth
>Laboratoire d'orthopedie de l'UCL
>Avenue Mounier 53, Tour Pasteur, 5388
>1200 Bruxelles
>
>jungbluth at orto.ucl.ac.be
>
1st: To inactivate the complement factors C2 and C4. 
Complement activation can damage your cultured cells.

2nd: Heating serum for 30 minutes at 56 degrees Celcius will
kill (if present) mycoplasmas.

Kees



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