Help needed with in situ hybridisation
iksaka at CC.JYU.FI
Thu Jun 6 10:13:07 EST 1996
Is there anyone who has been doing in situ hybridisation with the
instruction from Wisden and Morris's "In Situ Hybridisation Protocols for
the Brain", chapter one: ISH with synthetic oligonucleotide probes ?
I tried it once, but I ran into some problems:
1) The exposure time was pretty long, 3-4 weeks. I've heard that
proteinase K -treatment would help, anyone tried that ?
2) There was pretty much non-specific background. Is that just because of
the long exposure time or should I use acetic anhydride and chloroform
I used uninduced and unfixed brain and 45-mer c-fos probe.
I would be very thankful for any advice,
University of Kuopio
More information about the Cellbiol