CYCLOHEXIMIDE & Protein synthesis INHIBITION!

[Luc Nieland]

Dr.M.M.Rajan wrote:
> 
> Dear Reader,
>         I am working on Neuroblastoma differentiation and Apoptosis.
>         I would like to find out the optimal protein synthesis inhibitory
> concentrations of cycloheximide, which is also non toxic.
> 
> I have been using tritiated proline incorporation assay, to determine
> this, but the non-toxic concentrations are not inhibitory to protein
> synthesis.
> 
> Thanking you in anticipation
> Yours
> Dr.Rathna
> National Centre for Cell Science
> INDIA

For MR65 cells 500 ug/ml was used by a colleague of mine. This concentration
induces apoptosis. (Eur J Cell Biol 68:35-46 (1995)


Luc



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