mbxfd at unix.ccc.nottingham.ac.uk
Tue Jun 11 06:29:24 EST 1996
Can anyone point me to a good recent ref or review on subcellular
fractionation of yeast?
I'm thinking of replacing a liver fract with a yeast one for a class - so I
need to be able to get all the major organelles reasonably separated with
one fractionation (because other parts of the class look at yeast alcohol
dehydrogenase - so it makes sense). Advice on the need to prepare
spheroplasts or not appreciated.
Also, for research purposes a good way to prepare highly purified vacuoles.
All from S. cerevisiae, most probably.
University Medical School,
Queen's Medical Centre,
Nottingham NG7 2UH
Tel: (0)115 970 9366 FAX (0)115 942 2225 Internet:
Fergus.Doherty at nott.ac.uk
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