Subfractionating yeast

[Fergus Doherty]

Can anyone point me to a good recent ref or review on subcellular
fractionation of yeast?

I'm thinking of replacing a liver fract with a yeast one for a class - so I
need to be able to get all the major organelles reasonably separated with
one fractionation (because other parts of the class look at yeast alcohol
dehydrogenase - so it makes sense).  Advice on the need to prepare
spheroplasts or not appreciated.

Also, for research purposes a good way to prepare highly purified vacuoles.

All from S. cerevisiae, most probably.

Fergus Doherty,
dept. Biochemistry,
University Medical School,
Queen's Medical Centre,
Nottingham NG7 2UH
Tel: (0)115 970 9366  FAX (0)115 942 2225 Internet:
Fergus.Doherty at nott.ac.uk