CYCLOHEXIMIDE & Protein synthesis INHIBITION!

Richard A. Lockshin yo_doc at usa.pipeline.com
Tue Jun 11 09:14:51 EST 1996


See my reply sent to bionet.general 
 
On Jun 11, 1996 10:48:55 in article <Re: CYCLOHEXIMIDE & Protein synthesis
INHIBITION!>, 'Luc Nieland <lucn at molcelb0005.rulimburg.nl>' wrote: 
 
 
>Dr.M.M.Rajan wrote: 
>>  
>> Dear Reader, 
>>         I am working on Neuroblastoma differentiation and Apoptosis. 
>>         I would like to find out the optimal protein synthesis
inhibitory 
>> concentrations of cycloheximide, which is also non toxic. 
>>  
>> I have been using tritiated proline incorporation assay, to determine 
>> this, but the non-toxic concentrations are not inhibitory to protein 
>> synthesis. 
>>  
>> Thanking you in anticipation 
>> Yours 
>> Dr.Rathna 
>> National Centre for Cell Science 
>> INDIA 
> 
>For MR65 cells 500 ug/ml was used by a colleague of mine. This
concentration 
>induces apoptosis. (Eur J Cell Biol 68:35-46 (1995) 
> 
> 
>Luc 
> 
> 
> 
>+--------------------------------------------------------------------+ 
>| luc.nieland at molcelb.rulimburg.nl            Mol.Cell Biology,  RL  | 
>| room: 5.214 -> phone: (043-3881357)         FacII, Uns. 50         | 
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-- 
 
Richard A. Lockshin 




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