Subfractionating yeast

[Ricardo Azpiroz]

Actually, yeast mitochondria and nuclei are a cinch to purify.


On 12 Jun 1996 group at mendel.berkeley.edu wrote:

> In article <mbxfd-110696123054 at wpbfd.biochem.nottingham.ac.uk>,
> mbxfd at unix.ccc.nottingham.ac.uk (Fergus Doherty) wrote:
> 
> > Can anyone point me to a good recent ref or review on subcellular
> > fractionation of yeast?
> > 
> > I'm thinking of replacing a liver fract with a yeast one for a class - so I
> > need to be able to get all the major organelles reasonably separated with
> > one fractionation (because other parts of the class look at yeast alcohol
> > dehydrogenase - so it makes sense).  Advice on the need to prepare
> > spheroplasts or not appreciated.
> > 
> > Also, for research purposes a good way to prepare highly purified vacuoles.
> > 
> > All from S. cerevisiae, most probably.
> 
> 
> Bad news.  Yeast organelles are not easier to fractionate.  
> 
> Do a medline search on Peter Novick.  Look for a Methods in Enzymology or
> Methods in Cell Biology paper called    
> "Fractionating Yeast Organelles".
> 
> However, good protocols for purification of vacuoles exist.  Try a medline
> search on William Wickner.  He has a recent Cell paper on vacuole fusion.
> 
> 

----------------------
Ricardo Azpiroz
Department of Biochemistry
Life Sciences South
University of Arizona
Tucson AZ 85721-0106

azpiroz at U.Arizona.EDU