retina nuclear extract

[Jim Hutchins]

UYYIUGYJHJ (JHIVHGJ) wrote:
: Dear netters:
: I have a problem with nuclear extraction from rat retinas. When come to 
: homogenize the nuclei, there is always a jelly stuff formed and I can 
: not spin the supernatant out. (it's wrapped in that jelly stuff). Any 
: suggestion on this? 
: Thanx in advance.
: 
: Lihua He


As far as I know, that 'jelly stuff' *IS* DNA.  I'm not clear on what it
is you are trying to do.  Are you trying to extract the nuclei semi-intact
for a nuclear run-on, or what?

-- 
Jim Hutchins    hutchins at netdoor.com   hutchins at umsmed.edu
http://fiona.umsmed.edu/~hutchins/
Annie the Aust Cattle Dog // Kodiak the Norwegian Elkhound
"Morde diem" ****** Annie's motto ****** "Bite the Day"