retina nuclear extract
hutchins at netdoor.com
Sat Jun 15 11:36:02 EST 1996
UYYIUGYJHJ (JHIVHGJ) wrote:
: Dear netters:
: I have a problem with nuclear extraction from rat retinas. When come to
: homogenize the nuclei, there is always a jelly stuff formed and I can
: not spin the supernatant out. (it's wrapped in that jelly stuff). Any
: suggestion on this?
: Thanx in advance.
: Lihua He
As far as I know, that 'jelly stuff' *IS* DNA. I'm not clear on what it
is you are trying to do. Are you trying to extract the nuclei semi-intact
for a nuclear run-on, or what?
Jim Hutchins hutchins at netdoor.com hutchins at umsmed.edu
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