# calculations

Mon Jun 17 08:43:14 EST 1996

```Could anyone help me with a question.
My problem is I have a positive result from ELISA and a background
result.  I would like to subtract my background result from the
positive result.  These are two means of an average of two wells.  I
would like to take into account in this calculation the deviation
within each mean, for the positive and negative results, so as to
have a more representative result of the variation.  This I would
like to do by weighting the resulting value from the subtraction of
the two means by the standard deviation (or error of the two means).
Is such a calculation possible.
Is this clear !!!!!! I hope so statistics is not my field.
Thanks for any possible ideas.

Luke Scicluna

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`

```