cell energy status, methods

Andreas Jordan jordan at ukrv.de
Thu Jun 20 07:14:25 EST 1996


Hello HPLC freaks !

Does anyone has a reference of a HPLC method for quantification of all
relevant NTP,NDP, NMP and other energy metabolites from mammalian
cells from one FN-biopsy sample ? What is the best method to harvest
epithelial adherent cells to obtain a quite realistic cell energy
status for further HPLC analysis ? What are alternative methods to
quantify the cell energy status (except bioluminiscence) ?

Thanks so much in advance.



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