Assay for Protein kinase C in immuno-precipitates

[Helfried Mallow]

High, 
I need detailed lab protocols for an assay of protein kinase C  in 
immuno-preciptitates. This assay should be suitable for isoforms alpha and 
epsilon. Furthermore it should show a dependence of kinase activity on DOG or 
PMA. Detailed lab protocols for cell lysis, immunoprecipitation and kinase 
assay would be an great advantage for me

My e-mail number is :  manfred.maier at vkl.uni-regensburg.de

Thanks a lot for your efforts

Manfred Maier

PS. Could you tell me in which country do you work and what kind of text 
software do you use (Company; Version; and what kind of computers, Mac or IBM)