Collagen gels for Tissue Transplantation!

verlooy at uia.ua.ac.be verlooy at uia.ua.ac.be
Fri Jun 21 10:48:10 EST 1996


In article <Pine.EPX.3.92.960621093445.18519C-100000 at powai.cc.iitb.ernet.i, 
"Dr.M.M.Rajan" <mmrajan at cc.iitb.ernet.in> writes:

>
>Dear Researcher,
>	Hello!
>	I have been using collagen gels for the creation of a composite
>membrane in which I seed cells towards the study of cells for tissue
>transplantation.
>	Recently I started using UV exposure for sterilisation of the
>gels before seeding the cells. There appears to be change in cells
>adhesion to the gel. I am using the conventional UV hood which is used for
>crosslinking DNA to nylon membranes.
>	DOes anybody know whether there could be a change in cell adhesion
>by UV exposure.
>
>
>Also, any suggestions about the quantification of cell proliferations and
>growth in matrices of this sort. I have been using the MTT but it appears
>to be a bit difficult. There appears to be a delay in the crystal
>formation and extracting the crystals out. Should I use collagenase to
>lyse the matrix and release the cells out? Does this process affect the
>cell count?
>Help needed in these quarters...
>Thanking you in anticipation,
>Yours
>M.M.Rajan
>
>



Dear Dr. Rajan,



Perhaps you could try the Alamar Blue assay to determine the cell proliferation 
in your culture system.

Ref. S. Ansar Ahmed;  J. Immunol. Methods; 170 (1994) 211-224

For any further questions

Joris VERLOOY
Dept Pediatrics
University of Antwerp (U.I.A.)
Belgium

verlooy at uia.ua.ac.be
 



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