Sheet formation by trypsinized cells?

Anne Exton Stone aes2 at
Fri Jun 21 15:42:28 EST 1996

On 20 Jun 1996, Anthony Otsuka wrote:

We routinely trypsinize our cells to pass them and yes, they come up like 
sheets...I have no experience with mouse thymic epithelial cells. 

Gentle pipeting with a plastic 10 ml pipette against the bottom of the 
dish helps break up the aggregates.

hope this helps,


>     We have observed the formation of sheet-like structures when
> adding trypsin to mouse thymic epithelial cells in culture.  We
> were wondering if anyone working with these or other cell types
> have observed this aggregation phenomenon.  If you have, please
> send information on cell lines and your observations to
> nmnikol at  Thank you, Nikolaos Nikolaidis

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