Sheet formation by trypsinized cells?

[Anne Exton Stone]

On 20 Jun 1996, Anthony Otsuka wrote:


We routinely trypsinize our cells to pass them and yes, they come up like 
sheets...I have no experience with mouse thymic epithelial cells. 

Gentle pipeting with a plastic 10 ml pipette against the bottom of the 
dish helps break up the aggregates.

hope this helps,

regards,
anne





>     We have observed the formation of sheet-like structures when
> adding trypsin to mouse thymic epithelial cells in culture.  We
> were wondering if anyone working with these or other cell types
> have observed this aggregation phenomenon.  If you have, please
> send information on cell lines and your observations to
> nmnikol at rs6000.cmp.ilstu.edu.  Thank you, Nikolaos Nikolaidis
> 
>