Has anybody good experience with stable co-transfection in 3T3 fibroblasts
helfried.mallow at vkl.uni-regensburg.de
Thu Jun 20 11:18:43 EST 1996
I need someone who has good experience in stable transfection of 3T3
Problem : I have to transfect two constructs into 3T3-L1 preadipocytes
(fibroblasts). One repressor construct of 6.7 kb and and expression construct
of 7.8 or 8.8 kb. Transfection should be performed with Maxifectin, a liposome
Difficulty: I have not much time to find out detailed and optimal transfection
conditions. I need an established method, in order to save time and get a
satisfactory transfection efficiency.
Selection has to be performed with Hygromycin B and G418
Special questions : What are the optimal concentrations of those two
antibiotics in a double selection? How long will the selection last? I am
working in a serum-based system.
I am an absolute beginner referring to transfection.
My e-mail adress is : manfred.maier at vkl.uni-regensburg.de
If you or somebody else in your environment has got experience, please contact
Thanks a lot for your efforts
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