Azidopene binding and pgp
ggrant at umabnet.ab.umd.edu
Fri Jun 21 10:39:11 EST 1996
I have been working using a [3H] azidopine binding assay to test binding to
P-glycoprotien. However, I get no signal, only a wave of radioactivity on the
end of my gel, nothing arround the 170 mark.
My membranes seem fine, giving me a nice clean band on western analysis
Has anyone any ideas on what I may be doing wrong?
Thanks in advance
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