Azidopene binding and pgp

Geri Grant ggrant at
Fri Jun 21 10:39:11 EST 1996

I have been working using a [3H] azidopine binding assay to test binding to 
P-glycoprotien.  However, I get no signal, only a wave of radioactivity on the 
end of my gel, nothing arround the 170 mark.
My membranes seem fine, giving me a nice clean band on western analysis 
using C219.
Has anyone any ideas on what I may be doing wrong?

Thanks in advance 


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