single-strand DNA break detection

Scott Selby Med-Hem selby at hiv.med.umn.edu
Wed Mar 6 13:17:03 EST 1996


I'm wondering if anyone could direct me toward proteins that might be
involved in recognition of single-stranded DNA breaks (SSB).  The cell
line I'm working with has a decrease response to single-stranded DNA
damage, as evidenced by Western blottig, of p53.  The p53 is wild-type,
and I'm curious as to whether the defect is in a regulatory region of p53
upstream somewhere, or whether the actual detection of single-stranded
breaks is faulty.  Does anyone have knowledge as to what proteins are
specifically involved in detection SSBs? 

I'd love to hear any discussion about other possible causes of this muted 
p53 response.  Thanks, in advance.

______________________________________________________________________________
    ||         Scott A. Selby               6098)o%:::%o(860
   ====         Univ. of Minnesota          098)o%:::%o(8609
   |  |__        Dept. of Med/Hematology     6o%:%o(86098)
   |  |-.\        Box 480 UMHC                (86098)o
   |__|  \\        Minneapolis, MN  55455   6098)o%::%o9  
    ||   ||                                 098)o%::::::%o9
  ======__|        selby at lenti.med.umn.edu   6o%::::::%o(860
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