cell splitting problem
Unknown
Unknown
Tue Mar 12 05:34:41 EST 1996
I have a problem passaging some chick fibroblast (OU2) cells. When I use
the recommended mix of trypsin/edta, the cells come off as a single sheet
that does not want to split up, even if the cells are not yet confluent
(though with lots of intercell connections). Anyone ever experienced this
as a problem with any other cells, and overcome it? If so, how?
thanks for any help you can give
michael baron
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