cell splitting problem
David Ward Rusnak
rusnak~dw at glaxo.com
Tue Mar 12 08:06:15 EST 1996
In article <4i3k01$b79 at is.bbsrc.ac.uk>, michael.baron at bbsrc.ac.uk wrote:
> I have a problem passaging some chick fibroblast (OU2) cells. When I use
> the recommended mix of trypsin/edta, the cells come off as a single sheet
> that does not want to split up, even if the cells are not yet confluent
> (though with lots of intercell connections). Anyone ever experienced this
> as a problem with any other cells, and overcome it? If so, how?
> thanks for any help you can give
> michael baron
I had this happen to me once and I am not sure that this is your problem,
but here goes...
I was growing NIH3T3 cells and plated them in 100mm dishes. The next day
I went to look at them and they were in monolayer, but sliding in sheets
on the dish. I found out that I had plated on non-treated petri dishes in
stead of tissue culture dishes.
Could that be it??
I doubt it, but anyway...
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