cell splitting problem

David Aldridge aldridge at petermac.unimelb.edu.au
Wed Mar 13 21:52:53 EST 1996


In article <4i3k01$b79 at is.bbsrc.ac.uk>, michael.baron at bbsrc.ac.uk wrote:

> I have a problem passaging some chick fibroblast (OU2) cells. When I use
> the recommended mix of trypsin/edta, the cells come off as a single sheet 
> that does not want to split up, even if the cells are not yet confluent 
> (though with lots of intercell connections). Anyone ever experienced this 
> as a problem with any other cells, and overcome it? If so, how?
> 
> thanks for any help you can give
> 
> michael baron

You could try using a different protease such as pronase. Just be sure to
wash the cells well before replating as there is no pronase inhibitor in
serum.
Good luck



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