Animal liver cell cultures
m.j.gardner at bham.ac.uk
Thu Mar 14 17:10:55 EST 1996
Adrian J. Terranova wrote:
> I am looking for liver cell cultures for the following animals
> I need any and all relevent information on keeping the cells alive and
> reproducing and HAPPY!!!!!
> Any intro cell culture information would be greatly appreciated, any
> leads would also be greatly appreciated.
I have cultured primary rat hepatocytes on type I collagen coated TC
plates using Williams E medium and a 5% CO2 atmosphere.
However the cells only survive for less than a week and so you may not
consider them very "happy". They proliferate in the presence of mitogens
(eg EGF or HGF).
Hope this is of some help,
More information about the Cellbiol