Fixation of Endothelial Cells

Michael Daws Michael.Daws at
Fri Mar 22 11:23:01 EST 1996


I have used a 50:50 acetone methanol mix to fix epithelial cells to 
plastic.  I was looking at growth factor receptors, and found that this 
treatment still allowed antibody recognition of the receptor, but 
destroyed the ligand binding site.  Therefore I suspect this treatment 
may be a little harsh for your purposes.  Hope this helps


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