Neutral Red staining..Help!!
swalsh at bme.jhu.edu
Wed Mar 27 09:57:57 EST 1996
I am a grdauate student at Johns Hopkins looking for some insight on fluorescence
staining. I am trying to stain for endocytotic pathways and using Neutral red to
stain acidic organelles and Hoecsht to stain the Nucleus. The problem is..when I
am looking through a fluorescence microscope using a filter for Texas red...the
nucleus is also lighting up orange-red along with other areas of the cell
(presumably endo-lysosomes)! The catalog of Molecular probe Inc. doesn't
mention that Neutral red stains Nucleus also...Please..if any of you have any
experience with such staining and can provide me with some insights I will be
very grateful. Please e-mail at
kroy at bme.jhu.edu
Thanks a lot...
Johns Hopkins School of Medicine
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